Ribonuclease A CAS:9001-99-4

  • Product Name:Ribonuclease A
  • CAS:9001-99-4
  • Molecular formula:NULL
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COA:Downl oad MSDS:Downl oad

Ribonuclease A CAS:9001-99-4

Ribonuclease A CAS:9001-99-4 Basic information

Product Name: Ribonuclease A
Synonyms: Ribonuclease A from?Bovine pancreas, ≥ 60Kunitz units/mg;TBS BUFFER 20X LIQUID ULTRA PURE GRADE;WRIGHT S STAIN CERTIFIABLE GRADE;RNase A Solution(100mg/ml);Ribonuclease A from bovine pancreas(Purified Solution);Ribonuclease A from bovine pancreas(DNase &
CAS: 9001-99-4
MF: NULL
EINECS: 232-646-6
Product Categories: Nucleic acids;Enzyme Class Index;3.x.x.x Hydrolases;Others;General Metabolic Enzymes;Metabolomics;Nucleotide Metabolism
Mol File: Mol File

Ribonuclease A CAS:9001-99-4 Chemical Properties

Melting point: 243 °C (dec.)
form: solid
storage temp.: 2-8°C
Water Solubility: Soluble in water.
solubility: Reconstitute in 10mM acetate, pH 5.0 with or without 15mM calcium chloride
Sensitive: Moisture Sensitive
PH: D25 (per mg N) -0.47° (c = 5)
alpha: D25 (per mg N) -0.47° (c = 5)
EPA Substance Registry System: Nuclease, ribo- (9001-99-4)


TEST

 

SPECIFICATION

 

ANALYSIS

Appearance

Activity(Kunitz, Protein @ 37°C) Solubility (1%, Water)

White to light beige powder

≥60.0U/mg Confirmed

PASS

162U/mg PASS

Ribonuclease A Usage And Synthesis
Chemical PropertiesLyophilized
Purification MethodsPurify it by (NH4)2SO4 fractionation, followed by PC cellulose chromatography and affinity chromatography (using Sepharose 4B to which (G)n is covalently bonded). [Schmukler et al. J Biol Chem 250 2206 1975.] Ribozymes. These are ribonucleic acids which act like protein enzyme in catalyzing the making and breaking of peptide bonds as well as catalyzing reactions and cleavage of DNA and RNA molecules. The short RNAs are being intensively studied (see RNAi below). MicroRNAs (miRNA, see also below) are ubiquitous and are also genetically produced. They are involved in numerous reactions from splicing RNA, e.g. mRNA (messenger RNA) to controlling transcription of DNA to RNA, and translation of RNA to protein. Each miRNA is capable of being involved in a small number to hundreds of interactions with nucleic acids and with proteins.


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